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akt inhibitor  (MedChemExpress)
95
MedChemExpress akt inhibitor
E2 and VitD3 improves osteogenesis by <t>PI3K/AKT/mTOR</t> pathway. (A) Q-PCR analysis of mRNA level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (B) The protein level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (C) The WB analysis of protein level of pan and phsopho-PI3K, pan and phospho-Akt, pan and phospho-mTOR, and pan and phospho-FOXO3 in MC3T3-E1 cells treated with PBS, E2, VitD3, E2 plus VitD3. (D) The MC3T3-E1 cells were pretreated with or <t>without</t> <t>Akti1/2</t> (5uM), and were induced to osteoblasts with PBS, E2, VitD3, E2+VitD3. The ALP staining was utilized to evaluate the osteogenic differentiation in each group.
Akt Inhibitor, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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akt1 inhibitor  (MedChemExpress)
95
MedChemExpress akt1 inhibitor
E2 and VitD3 improves osteogenesis by <t>PI3K/AKT/mTOR</t> pathway. (A) Q-PCR analysis of mRNA level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (B) The protein level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (C) The WB analysis of protein level of pan and phsopho-PI3K, pan and phospho-Akt, pan and phospho-mTOR, and pan and phospho-FOXO3 in MC3T3-E1 cells treated with PBS, E2, VitD3, E2 plus VitD3. (D) The MC3T3-E1 cells were pretreated with or <t>without</t> <t>Akti1/2</t> (5uM), and were induced to osteoblasts with PBS, E2, VitD3, E2+VitD3. The ALP staining was utilized to evaluate the osteogenic differentiation in each group.
Akt1 Inhibitor, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/akt1 inhibitor/product/MedChemExpress
Average 95 stars, based on 1 article reviews
akt1 inhibitor - by Bioz Stars, 2026-02
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akt inhibitor ipatasertib  (MedChemExpress)
95
MedChemExpress akt inhibitor ipatasertib
E2 and VitD3 improves osteogenesis by <t>PI3K/AKT/mTOR</t> pathway. (A) Q-PCR analysis of mRNA level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (B) The protein level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (C) The WB analysis of protein level of pan and phsopho-PI3K, pan and phospho-Akt, pan and phospho-mTOR, and pan and phospho-FOXO3 in MC3T3-E1 cells treated with PBS, E2, VitD3, E2 plus VitD3. (D) The MC3T3-E1 cells were pretreated with or <t>without</t> <t>Akti1/2</t> (5uM), and were induced to osteoblasts with PBS, E2, VitD3, E2+VitD3. The ALP staining was utilized to evaluate the osteogenic differentiation in each group.
Akt Inhibitor Ipatasertib, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/akt inhibitor ipatasertib/product/MedChemExpress
Average 95 stars, based on 1 article reviews
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pi3k akt 339 pathway inhibitor ly294002  (MedChemExpress)
97
MedChemExpress pi3k akt 339 pathway inhibitor ly294002
E2 and VitD3 improves osteogenesis by <t>PI3K/AKT/mTOR</t> pathway. (A) Q-PCR analysis of mRNA level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (B) The protein level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (C) The WB analysis of protein level of pan and phsopho-PI3K, pan and phospho-Akt, pan and phospho-mTOR, and pan and phospho-FOXO3 in MC3T3-E1 cells treated with PBS, E2, VitD3, E2 plus VitD3. (D) The MC3T3-E1 cells were pretreated with or <t>without</t> <t>Akti1/2</t> (5uM), and were induced to osteoblasts with PBS, E2, VitD3, E2+VitD3. The ALP staining was utilized to evaluate the osteogenic differentiation in each group.
Pi3k Akt 339 Pathway Inhibitor Ly294002, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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akt kinase inhibitor  (MedChemExpress)
94
MedChemExpress akt kinase inhibitor
E2 and VitD3 improves osteogenesis by <t>PI3K/AKT/mTOR</t> pathway. (A) Q-PCR analysis of mRNA level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (B) The protein level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (C) The WB analysis of protein level of pan and phsopho-PI3K, pan and phospho-Akt, pan and phospho-mTOR, and pan and phospho-FOXO3 in MC3T3-E1 cells treated with PBS, E2, VitD3, E2 plus VitD3. (D) The MC3T3-E1 cells were pretreated with or <t>without</t> <t>Akti1/2</t> (5uM), and were induced to osteoblasts with PBS, E2, VitD3, E2+VitD3. The ALP staining was utilized to evaluate the osteogenic differentiation in each group.
Akt Kinase Inhibitor, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p akt inhibitor mk2206  (MedChemExpress)
96
MedChemExpress p akt inhibitor mk2206
E2 and VitD3 improves osteogenesis by <t>PI3K/AKT/mTOR</t> pathway. (A) Q-PCR analysis of mRNA level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (B) The protein level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (C) The WB analysis of protein level of pan and phsopho-PI3K, pan and phospho-Akt, pan and phospho-mTOR, and pan and phospho-FOXO3 in MC3T3-E1 cells treated with PBS, E2, VitD3, E2 plus VitD3. (D) The MC3T3-E1 cells were pretreated with or <t>without</t> <t>Akti1/2</t> (5uM), and were induced to osteoblasts with PBS, E2, VitD3, E2+VitD3. The ALP staining was utilized to evaluate the osteogenic differentiation in each group.
P Akt Inhibitor Mk2206, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p akt agonist sc79  (MedChemExpress)
96
MedChemExpress p akt agonist sc79
E2 and VitD3 improves osteogenesis by <t>PI3K/AKT/mTOR</t> pathway. (A) Q-PCR analysis of mRNA level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (B) The protein level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (C) The WB analysis of protein level of pan and phsopho-PI3K, pan and phospho-Akt, pan and phospho-mTOR, and pan and phospho-FOXO3 in MC3T3-E1 cells treated with PBS, E2, VitD3, E2 plus VitD3. (D) The MC3T3-E1 cells were pretreated with or <t>without</t> <t>Akti1/2</t> (5uM), and were induced to osteoblasts with PBS, E2, VitD3, E2+VitD3. The ALP staining was utilized to evaluate the osteogenic differentiation in each group.
P Akt Agonist Sc79, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p akt agonist sc79 - by Bioz Stars, 2026-02
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akt phosphorylation inhibitor ly294002  (MedChemExpress)
96
MedChemExpress akt phosphorylation inhibitor ly294002
<t>LY294002</t> suppresses exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells -induced angiogenesis. b P < 0.01; c P < 0.001. Data are presented as mean ± SD ( n = 3). A and B: Western blot analysis of phosphorylated protein kinase B, protein kinase B, hypoxia-inducible factor-1α, and vascular endothelial growth factor-A protein expression levels in human umbilical vein endothelial cells (HUVECs) treated with LY294002 vs negative control and LY294002 + exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells vs exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells; C and D: 5-Ethynyl-2’-deoxyuridine incorporation assay to assess HUVEC proliferation. Scale bar = 100 μm; E: Cell Counting Kit-8 assay to evaluate HUVEC viability; F and G: Transwell migration assay to assess the migratory capacity of HUVECs. Scale bar = 200 μm; H and I: In vitro wound healing assay to evaluate cell migration. Scale bar = 200 μm; J and K: Tube formation assay to examine the formation of capillary-like structures by HUVECs. Scale bar = 200 μm. EdU: 5-Ethynyl-2’-deoxyuridine; NC: Negative control; CO 2 laser-Exos: Exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells; AKT: Protein kinase B; p-AKT: Phosphorylated protein kinase B; HIF-1α: Hypoxia-inducible factor-1α; VEGF-A: Vascular endothelial growth factor-A.
Akt Phosphorylation Inhibitor Ly294002, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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E2 and VitD3 improves osteogenesis by PI3K/AKT/mTOR pathway. (A) Q-PCR analysis of mRNA level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (B) The protein level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (C) The WB analysis of protein level of pan and phsopho-PI3K, pan and phospho-Akt, pan and phospho-mTOR, and pan and phospho-FOXO3 in MC3T3-E1 cells treated with PBS, E2, VitD3, E2 plus VitD3. (D) The MC3T3-E1 cells were pretreated with or without Akti1/2 (5uM), and were induced to osteoblasts with PBS, E2, VitD3, E2+VitD3. The ALP staining was utilized to evaluate the osteogenic differentiation in each group.

Journal: Materials Today Bio

Article Title: Alendronic acid modified PLGA drug delivery system loaded with 17β-Estradiol and vitamin D3 has anti-osteoporotic effect

doi: 10.1016/j.mtbio.2026.102789

Figure Lengend Snippet: E2 and VitD3 improves osteogenesis by PI3K/AKT/mTOR pathway. (A) Q-PCR analysis of mRNA level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (B) The protein level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (C) The WB analysis of protein level of pan and phsopho-PI3K, pan and phospho-Akt, pan and phospho-mTOR, and pan and phospho-FOXO3 in MC3T3-E1 cells treated with PBS, E2, VitD3, E2 plus VitD3. (D) The MC3T3-E1 cells were pretreated with or without Akti1/2 (5uM), and were induced to osteoblasts with PBS, E2, VitD3, E2+VitD3. The ALP staining was utilized to evaluate the osteogenic differentiation in each group.

Article Snippet: The AKT inhibitor (Akti1/2, MedChemExpress, USA) was dissolved in DMSO and used at a final concentration of 10 μM.

Techniques: Staining

LY294002 suppresses exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells -induced angiogenesis. b P < 0.01; c P < 0.001. Data are presented as mean ± SD ( n = 3). A and B: Western blot analysis of phosphorylated protein kinase B, protein kinase B, hypoxia-inducible factor-1α, and vascular endothelial growth factor-A protein expression levels in human umbilical vein endothelial cells (HUVECs) treated with LY294002 vs negative control and LY294002 + exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells vs exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells; C and D: 5-Ethynyl-2’-deoxyuridine incorporation assay to assess HUVEC proliferation. Scale bar = 100 μm; E: Cell Counting Kit-8 assay to evaluate HUVEC viability; F and G: Transwell migration assay to assess the migratory capacity of HUVECs. Scale bar = 200 μm; H and I: In vitro wound healing assay to evaluate cell migration. Scale bar = 200 μm; J and K: Tube formation assay to examine the formation of capillary-like structures by HUVECs. Scale bar = 200 μm. EdU: 5-Ethynyl-2’-deoxyuridine; NC: Negative control; CO 2 laser-Exos: Exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells; AKT: Protein kinase B; p-AKT: Phosphorylated protein kinase B; HIF-1α: Hypoxia-inducible factor-1α; VEGF-A: Vascular endothelial growth factor-A.

Journal: World Journal of Diabetes

Article Title: Fractional carbon dioxide laser-induced photothermal activation of mesenchymal stem cell-derived exosomes accelerates diabetic wound healing by enhancing angiogenesis

doi: 10.4239/wjd.v17.i1.112942

Figure Lengend Snippet: LY294002 suppresses exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells -induced angiogenesis. b P < 0.01; c P < 0.001. Data are presented as mean ± SD ( n = 3). A and B: Western blot analysis of phosphorylated protein kinase B, protein kinase B, hypoxia-inducible factor-1α, and vascular endothelial growth factor-A protein expression levels in human umbilical vein endothelial cells (HUVECs) treated with LY294002 vs negative control and LY294002 + exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells vs exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells; C and D: 5-Ethynyl-2’-deoxyuridine incorporation assay to assess HUVEC proliferation. Scale bar = 100 μm; E: Cell Counting Kit-8 assay to evaluate HUVEC viability; F and G: Transwell migration assay to assess the migratory capacity of HUVECs. Scale bar = 200 μm; H and I: In vitro wound healing assay to evaluate cell migration. Scale bar = 200 μm; J and K: Tube formation assay to examine the formation of capillary-like structures by HUVECs. Scale bar = 200 μm. EdU: 5-Ethynyl-2’-deoxyuridine; NC: Negative control; CO 2 laser-Exos: Exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells; AKT: Protein kinase B; p-AKT: Phosphorylated protein kinase B; HIF-1α: Hypoxia-inducible factor-1α; VEGF-A: Vascular endothelial growth factor-A.

Article Snippet: To evaluate the involvement of the PI3K/AKT signaling pathway in CO 2 laser-Exos-mediated angiogenesis, HUVECs were pretreated with either the AKT phosphorylation inhibitor LY294002 or the activator SC79 (MedChem Express, United States).

Techniques: Derivative Assay, Western Blot, Expressing, Negative Control, Cell Counting, Transwell Migration Assay, In Vitro, Wound Healing Assay, Migration, Tube Formation Assay